Amebic dysentery Diagnosis

The diagnosis for Amebic dysentery, must be based on medical history and symptoms in patients. Also the important diagnosis is pathogene inspection, fecal check to amoeba pathogens as the only basis for a reliable diagnosis. Usually found large trophozoites in patients as Amebic dysentery, and found a small trophozoites or cysts as the only infected.

1. Stool examination:
1) Alive trophozoites test: direct saline smear method commonly used to check the activities of the trophozoites. Blood of patients with acute dysentery or amebic inflammation will looser stools patients requiring containers clean, fresh Fecal examination and the faster the better, the cold season, we must pay attention to delivery and inspection of the insulation. Check check a clean slide, dropping 1 drop of saline, and then a small amount of feces pro-bamboo pads, painted in the normal saline, add coverslip, and then placed under a microscope to check. Amoebic dysentery stool for a typical red sauce myxoid, fishiness. Microscopy can be seen with more viscous mucus in the integrated group and fewer red blood cells white blood cells, sometimes we can see the crystallization of Charcot-Leyden's (Charcot-Leyden crystals) and activities of the trophozoites. These features can be differentiated from the faeces of bacillary dysentery.

2) Cyst test: clinical smear method commonly used iodine solution, which is user-friendly. Take a clean glass slide, dropping 1 drop of iodine solution, and then a small amount of fecal samples bamboo pads, painted in the iodine solution in thin coverslip added, and then placed under a microscope to check to identify the characteristics and the number of nuclei .

2. Entamoeba culture: there are a variety of improved artificial medium, commonly used as Locke's solution, eggs, serum-free medium, serum saline nutrient agar medium, King lipoprotein biphasic medium such as peptone. However, the complexity of technical operations, to be certain equipment, and cultured Entamoeba in most cases of subacute or chronic positive rate is not high, it is not suitable for diagnosis of amebic wellness checks.

3. Biopsy: By Sigmoidoscopy or colonoscopy to observe fiber mucosal ulcers, and tissue biopsy or scraping smear swab material, the highest detection rate. It is reported that the sigmoid colon and rectum are the cases of lesions in patients with symptomatic or about 2 / 3, therefore, all patients with suspicious circumstances should be allowed to make the colonoscopy, scraping smear or swab from biopsy material. Trophozoites of the material must be the edge of ulcer, after the clamp from appropriate local slight bleeding. Vomica puncture fluid examination of characteristics in addition to the attention should be drawn from the Department of Vomica wall, made it easier for policy trophozoites.

4. Immunodiagnostics:
In recent years both at home and abroad have reported a variety of serological diagnostic methods, including indirect hemagglutination (IHA), indirect fluorescent antibody (IFAT) and enzyme-linked immunosorbent assay (ELISA) studies, it is sensitive to different cases of various types. IHA higher sensitivity of intestinal amoebiasis the positive rate was 98%, parenteral amebiasis positive rate of 95%, and asymptomatic carriage of only 10% ~ 40%, IFA sensitivity slightly than IHA. EALSA sensitive, specific high promising. Complement fixation test in the diagnosis of amebic also trap the meaning of things, the positive rate of up to 80%. Other factors, such as gelatin diffusion precipitin test, intradermal test, such as the value of both the diagnosis. In recent years, applications have been reported sensitive of immunology and pus in stool amebic antigen was detected to be successful. Noise in particular anti-tumor monoclonal antibody amoebic application of immunological techniques to detect pathogens in fecal material and host a reliable, sensitive and anti-jamming style of the tracer.