Intestinal amoebiasis is primarily parasitic in the colon, causing amoebic colitis or amoebic dysentery. Rhizopoda amebic dysentery is the most important insect pathogen types, under certain conditions, can be extended to liver, lung, brain, urinary and reproductive system and other parts of the body, the formation of ulcers and abscesses.
Morphological changes in Intestinal amoebiasis can be divided into trophozoites period, cyst early and cyst phase. The trophozoites period is divided into two types include large and small. The large trophozoites, live in the intestinal wall; small trophozoites live in the intestine, can form cysts. So the former, also known as tissue-type large trophozoites, the latter is also known as commensalism small trophozoites encysted.
1) large trophozoites: parasites in the tissue, the disease is enteropathy-type amoeba. The activities of large trophozoites larger volume, the diameter of 12 ~ 60μm, 15 ~ 30μm for the common. Since the beginning of its body outside the quality and quality, quality things transparent, all about 1 / 3, than the dense endoplasmic reticulum, were granular. Extend outside the quality movement, and often the formation of a single pseudopodium for targeted mobile campaigns that amoeba. First, extend outside the quality tongue or finger-like pseudopodia, then endoplasmic pseudopodium flows so that the whole body stretched out toward the direction of pseudopod movement was common, including the quality of its red blood cell phagocytosis. To post-stained with iron hematoxylin, showing a round nucleus, was bubbly, size 4 ~ 9μm, an average of 5.3μm, inner nuclear layer of uniform size, neatly arranged plasmid chromatin, the nuclear center of the circle with a small nucleolar form can also be a bit more, and between nucleolus and nuclear membrane can be seen in some cases nuclear fiber mesh.
2) small trophozoites: living in the small intestine of trophozoites to intestinal bacteria and intestinal contents for nutrition, not red blood cell phagocytosis, in the normal saline smear of smaller diameter of 12 ~ 30μm, the size of large trophozoites difficult to distinguish between, both inside and outside the boundaries of unknown quality, the Food and bacteria contained in the bubble. After stained with iron hematoxylin, the nucleus of trophozoites with the same structure.
2. Cyst early: it is small trophozoites form cysts in the transitional phase. Smaller size of this period, activity ceased, the metabolic reduction of cytoplasmic inclusions or discharged by the digestion disappear. Slight changes in the nucleus, the inner nuclear membrane particles than the aggregation of chromatin, nucleolus is slightly larger. Round in shape throughout the body.
3. Cyst: a round ball, the diameter of 5 ~ 20μm, wall thickness 0.5cm, transparent, highly refractive, not eosin staining. Microscope at low times, but the transparent circular body, the internal structure are not clear. In the high-power microscope, you can be chromosomal (chromatoid bodies) and nuclear circular reflector. When stained with iodine, the entire cyst shows brown, relatively clear, the Audit 1 to 4, occasionally 8. In the single-core or dual-core encapsulation, we see that the glycogen stain brown foam (glycogen vacuole), as well as to be transparent clavate chromosome (which may be poly ribosome). When the formation of mature infectious nuclear cyst 4, the chromosome and glycogen to be the gradual disappearance of the bubble. Stained with iron hematoxylin, the glycogen in the dyeing process of the bubble be dissolved into a vacuole, to be more clearly chromosomes, showing clavate, blunt at both ends of a round.